Plasmid

Part:BBa_K5322037:Design

Designed by: Xinquan Zhou   Group: iGEM24_NJTech-China   (2024-10-01)


pET29a-J23119-RBS-SoxR-T7-pSoxS-RBS-eGFP-T7


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 683
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 552
    Illegal NheI site found at 702
    Illegal NheI site found at 725
    Illegal NheI site found at 1475
    Illegal SpeI site found at 683
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 683
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 683
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization was performed according to the E. coli codon preference table.


Source

BBa_J23119 BBa_B0034 BBa_K554003 BBa_K731721 BBa_K5322004 BBa_B0034 BBa_K2807009 BBa_K731721

References